H Ishiwa and Escherichia coli with a minus origin . Shuttle plasmid vectors for Lactobacillus casei
نویسنده
چکیده
and Escherichia coli with a minus origin.
منابع مشابه
Development of an Escherichia coli-Lactobacillus casei shuttle vector for heterologous protein expression in Lactobacillus casei.
There is an increasing interest to develop various lactic acid bacteria (LAB) species as mucosal delivery vehicles, for which the development of a variety of cloning and expression systems for these bacteria is of primary importance. This study reports the complete nucleotide sequence of the cryptic plasmid pRCEID7.6 derived from the chicken probiotic LAB strain Lactobacillus casei TISTR1341. S...
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Three new Lactobacillus vectors based on cryptic Lactobacillus plasmids were constructed. The shuttle vector pLP3537 consists of a 2.3-kb plasmid from Lactobacillus pentosus MD353, an erythromycin resistance gene from Staphylococcus aureus plasmid pE194, and pUC19 as a replicon for Escherichia coli. The vectors pLPE317 and pLPE323, which do not contain E. coli sequences, were generated by intro...
متن کاملCharacterization of a theta-type plasmid from Lactobacillus sakei: a potential basis for low-copy-number vectors in lactobacilli.
The complete nucleotide sequence of the 13-kb plasmid pRV500, isolated from Lactobacillus sakei RV332, was determined. Sequence analysis enabled the identification of genes coding for a putative type I restriction-modification system, two genes coding for putative recombinases of the integrase family, and a region likely involved in replication. The structural features of this region, comprisin...
متن کامل“Direct cloning in Lactobacillus plantarum: Electroporation with non-methylated plasmid DNA enhances transformation efficiency and makes shuttle vectors obsolete”
BACKGROUND Lactic acid bacteria (LAB) play an important role in agricultural as well as industrial biotechnology. Development of improved LAB strains using e.g. library approaches is often limited by low transformation efficiencies wherefore one reason could be differences in the DNA methylation patterns between the Escherichia coli intermediate host for plasmid amplification and the final LAB ...
متن کاملHigh- and low-copy-number Lactococcus shuttle cloning vectors with features for clone screening.
High- and low-copy-number shuttle cloning vectors were constructed by incorporating the Escherichia coli P15A plasmid origin of replication into the pAM beta 1-derived vectors, pIL252 and pIL253. The resulting vectors were structurally stable in Lactococcus, which is a common feature of theta-replicating plasmids, and also displayed good structural stability in E. coli, possibly due to lack of ...
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تاریخ انتشار 1991